LAMB’S-QUARTERS INTERFERENCE WITH DIRECT SEEDED BROCCOLI

Direct microbial interference with xenobiotics

Introduction The human body is home to a complex microbial ecosystem. The gastrointestinal tract houses the most numerous microbial community (the gut microbiota), consisting of approximately 1013 microbial cells (1). Collectively, the aggregate genomes of the gut microbiota encode more than 100 times as many unique genes as the approximately 20,000 protein-coding genes found in the human genom...

Pantry, Kitchen & Servant's Quarters

Impact of Direct Seeded Rice (DSR) For Resource Conservation

Puddling doesn’t have much influence on rice yields. Many changes and innovations have contributed to the expanding use of resource conserving technologies in the country. One of the most important has been the development, testing and marketing of a low cost seed-cum-fertilizer drill which can establish crops with a minimum of soil disturbance, can take best advantage of residual soil moisture...

Direct freeform fabrication of seeded hydrogels in arbitrary geometries.

A major challenge in tissue engineering is the generation of cell-seeded implants with structures that mimic native tissue, both in anatomic geometries and intratissue cell distributions. By combining the strengths of injection molding tissue engineering with those of solid freeform fabrication (SFF), three-dimensional (3-D) pre-seeded implants were fabricated without custom-tooling, enabling e...

Rapid method for direct extraction of mRNA from seeded soils.

A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by vigorously shaking the washed soil in a 4 M guanidine thiocyanate solution containing 25 mM sodium citrate, 0.5% sarcosyl, and 0.1 M 2-mercaptoethanol. The pH of the homo...